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southern biotech cat n◦ 6160 05  (SouthernBiotech)


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    SouthernBiotech southern biotech cat n◦ 6160 05
    Southern Biotech Cat N◦ 6160 05, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rabbit+hrp+southern+biotech/Rabbit+Anti-Goat+IgG(H%2BL)-HRP/pm41746043-89-15-15
    Average 94 stars, based on 125 article reviews
    southern biotech cat n◦ 6160 05 - by Bioz Stars, 2026-10
    94/100 stars

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    Western Blot:

    Article Title: Post-inflammatory administration of N -acetylcysteine reduces inflammation and alters receptor levels in a cellular model of Parkinson's disease.
    Article Snippet: .. Source Dilution Antibody (primary) Lag3 (rabbit) Abcam (ab209236) 1/200 (IF) 1/1000 (WB) TLR2 (mouse) Abcam (ab16894) 1/200 (IF) 1/1000 (WB) α- Synuclein (mouse) Biolegend (807806) 1/1000 (IF) 1/2000 (WB) α- Synuclein (rabbit) Santa Cruz (sc- 7011) 1/200 (IF) α- Synuclein (rabbit) Cell Signaling (CST 2628) 1/400 (IF) IL- 1 beta (rabbit) Abcam (ab9722) 1/1000 (WB) TNF- α (rabbit) Cell Signaling (CST 3707) 1/1000 (WB) Caspase- 9 (rabbit) Cell Signaling (CST 9502) 1/1000 (WB) PARP (rabbit) Cell Signaling (CST 9542) 1/1000 (WB) Actin (rabbit) Sigma- Aldrich (A5060) 1/5000 (WB) Actin (mouse) Sigma- Aldrich (A5316) 1/5000 (WB) Vinculin (mouse) Sigma- Aldrich (V9131) 1/5000 (WB) Antibody (secondary) Alexa Fluor 488 (goat anti- mouse) Thermo Fisher Scientific (A11001) 1/500 (IF) Alexa Fluor 488 (goat anti- rabbit) Thermo Fisher Scientific (A11008) 1/500 (IF) Alexa Flour 568 (goat anti- mouse) Thermo Fisher Scientific (A11004) 1/500 (IF) Alexa Fluor 568 (goat anti- rabbit) Thermo Fisher Scientific (A11011) 1/500 (IF) Goat anti- mouse HRP Bio- Rad (101005) 1/5000 (WB) Goat anti- rabbit HRP Southern Biotech (4010- 05) 1/5000 (WB) Abbreviations: IL, interleukin; LAG3, lymphocyte- activation gene 3; TLR2, Toll- like receptor 2; TNF- α, tumor necrosis factor alpha. nloaded from https://faseb.onlinelibrary.w iley.com /doi/10.1096/fba.2022-00145 by IN A SP - N E PA L , W iley O nline L ibrary on [01/04/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Thermal cycler (Eppendorf Mastercycler Pro) under 10 min at 25°C, 120 min at 37°C, 5 min at 85°C, and ∞ at 4°C.

    Article Title: The VEGFR/PDGFR tyrosine kinase inhibitor, ABT-869, blocks necroptosis by targeting RIPK1 kinase
    Article Snippet: .. This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).674 D ow nloaded from http://portlandpress.com /biochem j/article-pdf/480/9/665/946420/bcj-2023-0035.pdf by guest on 16 D ecem ber 2023 Western Blot Primary Antibodies Protein Antibody Supplier Catalogue # Dilution Human RIPK1 Mouse anti-RIPK1 BD Transduction Laboratories cat #610458 1 : 1000 Human RIPK3 Rat anti-human RIPK3 clone 1H2 Produced in-house [9], available from Merck Millipore cat #MABC1640 1 : 1000 Phospho-mouse MLKL Rabbit anti-mouse MLKL pS345 Abcam cat #ab196436 1 : 1000 Phospho-mouse RIPK1 Rabbit anti-mouse RIPK1 pS166 Cell Signaling Technology cat #31122 1 : 1000 Phospho-mouse RIPK3 Rabbit anti-mouse RIPK3 pT231/pS232 Kindly supplied by Genentech, GEN135-35-9 [62] N/A 1 : 5000 Actin Chicken anti-β-actin-HRP Santa Cruz Biotechnology cat #sc-47778 HRP 1 : 5000 Western Blot Secondary Antibodies Antibody Supplier Catalogue # Dilution Goat anti-rat-HRP Southern Biotech cat #3010-05 1 : 5000 or 1 : 10 000 Goat anti-mouse-HRP Southern Biotech cat #1010-05 1 : 5000 or 1 : 10 000 Goat anti-rabbit-HRP Southern Biotech cat #4010-05 1 : 5000 or 1 : 10 000 Western blotting general procedure Proteins were transferred to nitrocellulose membranes using the iBlot Dry Blotting System (Invitrogen). ..

    Activation Assay:

    Article Title: Post-inflammatory administration of N -acetylcysteine reduces inflammation and alters receptor levels in a cellular model of Parkinson's disease.
    Article Snippet: .. Source Dilution Antibody (primary) Lag3 (rabbit) Abcam (ab209236) 1/200 (IF) 1/1000 (WB) TLR2 (mouse) Abcam (ab16894) 1/200 (IF) 1/1000 (WB) α- Synuclein (mouse) Biolegend (807806) 1/1000 (IF) 1/2000 (WB) α- Synuclein (rabbit) Santa Cruz (sc- 7011) 1/200 (IF) α- Synuclein (rabbit) Cell Signaling (CST 2628) 1/400 (IF) IL- 1 beta (rabbit) Abcam (ab9722) 1/1000 (WB) TNF- α (rabbit) Cell Signaling (CST 3707) 1/1000 (WB) Caspase- 9 (rabbit) Cell Signaling (CST 9502) 1/1000 (WB) PARP (rabbit) Cell Signaling (CST 9542) 1/1000 (WB) Actin (rabbit) Sigma- Aldrich (A5060) 1/5000 (WB) Actin (mouse) Sigma- Aldrich (A5316) 1/5000 (WB) Vinculin (mouse) Sigma- Aldrich (V9131) 1/5000 (WB) Antibody (secondary) Alexa Fluor 488 (goat anti- mouse) Thermo Fisher Scientific (A11001) 1/500 (IF) Alexa Fluor 488 (goat anti- rabbit) Thermo Fisher Scientific (A11008) 1/500 (IF) Alexa Flour 568 (goat anti- mouse) Thermo Fisher Scientific (A11004) 1/500 (IF) Alexa Fluor 568 (goat anti- rabbit) Thermo Fisher Scientific (A11011) 1/500 (IF) Goat anti- mouse HRP Bio- Rad (101005) 1/5000 (WB) Goat anti- rabbit HRP Southern Biotech (4010- 05) 1/5000 (WB) Abbreviations: IL, interleukin; LAG3, lymphocyte- activation gene 3; TLR2, Toll- like receptor 2; TNF- α, tumor necrosis factor alpha. nloaded from https://faseb.onlinelibrary.w iley.com /doi/10.1096/fba.2022-00145 by IN A SP - N E PA L , W iley O nline L ibrary on [01/04/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Thermal cycler (Eppendorf Mastercycler Pro) under 10 min at 25°C, 120 min at 37°C, 5 min at 85°C, and ∞ at 4°C.

    Produced:

    Article Title: The VEGFR/PDGFR tyrosine kinase inhibitor, ABT-869, blocks necroptosis by targeting RIPK1 kinase
    Article Snippet: .. This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).674 D ow nloaded from http://portlandpress.com /biochem j/article-pdf/480/9/665/946420/bcj-2023-0035.pdf by guest on 16 D ecem ber 2023 Western Blot Primary Antibodies Protein Antibody Supplier Catalogue # Dilution Human RIPK1 Mouse anti-RIPK1 BD Transduction Laboratories cat #610458 1 : 1000 Human RIPK3 Rat anti-human RIPK3 clone 1H2 Produced in-house [9], available from Merck Millipore cat #MABC1640 1 : 1000 Phospho-mouse MLKL Rabbit anti-mouse MLKL pS345 Abcam cat #ab196436 1 : 1000 Phospho-mouse RIPK1 Rabbit anti-mouse RIPK1 pS166 Cell Signaling Technology cat #31122 1 : 1000 Phospho-mouse RIPK3 Rabbit anti-mouse RIPK3 pT231/pS232 Kindly supplied by Genentech, GEN135-35-9 [62] N/A 1 : 5000 Actin Chicken anti-β-actin-HRP Santa Cruz Biotechnology cat #sc-47778 HRP 1 : 5000 Western Blot Secondary Antibodies Antibody Supplier Catalogue # Dilution Goat anti-rat-HRP Southern Biotech cat #3010-05 1 : 5000 or 1 : 10 000 Goat anti-mouse-HRP Southern Biotech cat #1010-05 1 : 5000 or 1 : 10 000 Goat anti-rabbit-HRP Southern Biotech cat #4010-05 1 : 5000 or 1 : 10 000 Western blotting general procedure Proteins were transferred to nitrocellulose membranes using the iBlot Dry Blotting System (Invitrogen). ..



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